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Collecting and sending invertebrate samples

Mites

Collecting Mites samples

  • Collect a bulk of >50 individual mites per paddock/area if possible
  • If possible, collect a bulk of >50 individual mites perpaddock/area.
  • Use a hard rigid plastic container. A container with a screw lid is best. To order a collection kit (container, labels and postage pack), contact Insect Factory at grahamhall1952@gmail.com or +267 71 777 467.
  • Place a layer of paper towel at the base of the plastic container.
  • Place a small amount of vegetation on top of the paper towel to cover it roughly.
  • Collect the mites from the paddock or area by tapping mite-infested vegetation over the plastic container so that they fall into it. If they are on the soil surface, you could try scooping them up with a spoon.
  • Take a piece of paper towel, position it over the opening of the plastic container, and securely close the lid on top of the paper towel. Make sure the paper towel is larger than the container to create a seal between the lid and the container, otherwise, mites may escape.
  • Wrap the plastic container in bubble wrap if you have some.

Aphids

Collecting Aphid samples

  • Collect a bulk of >50 individual aphids per paddock/area.
  • At each paddock/area, directly remove (cut)leaves that contain aphids, leaving the aphids undisturbed.
  • Collect a bulk of >50 individual aphids per paddock/area. ase do not use a take-away container or in zip lock bags as these can break or squash in the post.
  • Excessive plant matter can lead to high moisture levels, which may kill the aphids. Avoid over-packing the container with plant matter and place a piece of tissue paper or paper towel into the container to absorb excess moisture.

Moths and their parasitoids

  • The easiest way to collect moth samples in the field is by collecting the egg masses from the underside of the leaf. You can collect the larvae (caterpillars) and pupae which can be put in with the eggs.
  • Collecting a mixture of larvae, pupae, and eggs ensures high survival rates.
  • Collect a bulk of >20 individual larvae per paddock/area.
  • Aim to collect 50+ eggs from leaves and place them in a clear, non-crushable plastic container with some leaf material in case eggs hatch during transit. (The eggs are brittle so aim to keep the egg masses on the same leaf tissue you found them on).
  • You can add larval collections in the same container, and make sure there is enough vegetation for them to feed on while in transit.
  • Aim to collect 50+ eggs from leaves and place them in a clear, non-crushable plastic container with some leaf material in case eggs hatch during transit. (The eggs are brittle, so aim to keep the egg masses on the same leaf tissue you
  • Scrunch up some paper towels and add them to the container to absorb excess moisture.
  • Place a sheet of paper towel over the top of the container and close the lid over it.